In the research on the method of fractional precipitation, we mainly verify the possibility from theories to physically by experiment. 在非铁磁性磨粒的分步沉积方法的研究中,主要是通过实验来验证分步沉积法从理论到实际应用中的可行性。
Methods Urinary proteins were abstracted by fractional precipitation method with ammonium sulfate from nephrotic proteinuria of three patients with minimal change disease ( MCD). 方法用硫酸铵沉淀法提取肾小球微小病变患者尿液中的总蛋白成分。
METHODS By using techniques such as acid and alkaline extraction, ammonium sulfate fractional precipitation, membrane filtration and chromatographic separation, up to 9 kinds of enzymes were simultaneously extracted and purified from 1 kg of porcine pancreas in one process. 方法采用酸、碱抽提、硫酸铵分级沉淀、膜过滤、色谱分离等技术,从1kg猪胰脏中同时分离纯化9种酶。
Shark cartilage preparation ( SCP) was extracted from shark cartilage by the method of guanidine hydrochloride extraction, acetone fractional precipitation, and ultrafiltration. 以鲨鱼软骨为原料,经盐酸胍抽提,丙酮分级沉淀,超滤等步骤得到鲨鱼软骨制剂(sharkcartilagepreparation,SCP)。
Fractionation of humic acid by fractional precipitation technique 胡敏酸的酒精分级沉淀法分级
Modulation of Spinnable Polycarbosilane with a High Melting Point via Fractional Precipitation 沉淀分级法调制纺丝级高熔点聚碳硅烷
2) Three kinds of processes for extracting magnisium salt from salt lake water are re-commended,( a) the fractional crystallization processes,( b) lime precipitation processes, and ( c) ion exchange processes. 自盐湖水提取镁盐之方法,有三类,(1)分级结晶法类,(2)石灰沈降法类,(3)离子交换法类。
Chromatographically pure chitinase could be obtained by ( NH4) 2SO4 fractional precipitation, anion-exchange chromatography, SDS-PAGE, and a singular and high chromatography peak could be found and two electrophoretic bands could be validated from gel electrophoresis. 几丁质酶通过(NH4)2SO4分级沉淀、阴离子交换柱层析、SDS-PAGE提纯后可以达到层析纯,出现单一并且峰值很高的层析峰,电泳确认两条带,分别为几丁质外切酶和几丁质内切酶。
Obtain the crude enzyme solution by thermally denature earthworm tissue homogenate. Acquire the crude enzyme by ammonium sulfate fractional precipitation and 80% acetone precipitation. Purify the crude enzyme by ion exchange, hydrophobic exchange and gel filtration chromatography. 2. 双胸蚓组织匀浆后通过热变性获得粗酶液;利用硫酸铵分级沉淀和80%丙酮沉淀获得核酸酶的粗品;粗品依次通过离子交换层析、疏水交换层析和凝胶过滤层析进行精细纯化。